Pectin lyase production by recombinant Penicillium griseoroseum strain 105
نویسندگان
چکیده
منابع مشابه
Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression
Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants w...
متن کاملEnzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light...
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Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers ...
متن کاملProduction of Pectate Lyase by Penicillium viridicatum RFC3 in Solid-State and Submerged Fermentation
Pectate lyase (PL) was produced by the filamentous fungus Penicillium viridicatum RFC3 in solid-state cultures of a mixture of orange bagasse and wheat bran (1 : 1 w/w), or orange bagasse, wheat bran and sugarcane bagasse (1 : 1 : 0.5 w/w), and in a submerged liquid culture with orange bagasse and wheat bran (3%) as the carbon source. PL production was highest (1,500 U mL(-1) or 300 Ug(-1) of s...
متن کاملBiosynthesis of Acid Proteases by Penicillium Griseoroseum Ih-02 in Solid-state Fermentation
The present investigation describes the production of acid proteases by Penicillium griseoroseum IH-02 using solid-state fermentation technique. The enzyme biosynthesis was maximum (8.2 U/ml) when fermentation was carried out on a substrate containing soybean meal and wheat bran mixed at a ratio of 3:1 and moistened with D4 diluent. The substrate to diluent ratio was found to be optimum at 1:2....
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ژورنال
عنوان ژورنال: Canadian Journal of Microbiology
سال: 2010
ISSN: 0008-4166,1480-3275
DOI: 10.1139/w10-072